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    in vitro fertilisation (ivf)

    Steps Involved in IVF Treatment Process

    • 4.5   (1 Votes)

    In Vitro Fertilization or test tube baby is an Assisted Reproductive Technology procedure for infertile couples or individuals whereby eggs from a womans ovary are extracted for fertilization with sperms in a laboratory dish or test tube and the prepared embryos are then transferred to the uterus.

    What is IVF?

    In Vitro Fertilization treatment is a series of medical and surgical procedures performed to help couples combating with infertility, start their family. It is a form of Assisted Reproductive Technology (ART) procedures, commonly known as IVF. The IVF treatment process involves the extraction of eggs and retrieval of sperms and then combining eggs and sperms for the process of fertilization in a fertility laboratory. The prepared or fertilized egg is then transferred to the woman’s uterus. For years now, IVF treatments have been a popular option for couples who have been trying to conceive for one year or more. As per the statistics, more than one billion babies have been successfully born from In Vitro Fertilization treatment procedures. (1)

    Over the years the intervention of ART procedures infertility treatments have doubled the chances of pregnancy, thereby helping people with infertility disorders to enjoy parenthood. IVF treatment procedures are opted by individuals or couples trying to conceive after they have failed other ART procedures such as Intrauterine Insemination (IUI), oral medications, surgeries, and ovulation stimulation methods.

    Purpose of IVF Treatment Process

    Infertility is suspected for people who have been failing to achieve pregnancy for a period of twelve consecutive months or more despite having regular unprotected intercourse. Reasons for failed conceptions can be many. Some of the causes of female infertility are damaged or blocked fallopian tubes, endometriosis, and hormonal imbalance. In men, infertility can be due to low sperm count or poor quality sperm can cause infertility. IVF is one of several possible methods which helps increase the chance for infertile couples to achieve successful conception. IVF can be an option if there is a blockage in the fallopian tube or endometriosis in women or low sperm count or no sperm in men. Other possible treatments such as surgical procedures for blocked tubes or endometriosis are tried before IVF for these conditions. (2)

    Getting Started with IVF Treatment Process

    The process of IVF treatment begins on Day one of a female’s menstrual cycle and ends with egg retrieval. It is being told by the IVF specialists that the success rates of pregnancy get increased exponentially as more and more cycles are performed. In the first phase of the IVF treatment process, a woman is given birth control pills for hormone regulation and on day 21, she is given ovary stimulation medicines for ten days. The stimulation medications help the pituitary gland to secrete multiple follicle-stimulating hormones (FSH), instead of the single one which occurs during the natural cycle. This starts the development of ovarian follicles containing eggs. The woman undergoes ultrasound monitoring and other blood tests during the stimulation period, which lasts up to 12 days. (3)

    IVF Treatment Procedure Step by Step

    Medications and injections are started prior to the first day of the cycle as a fertility treatment plan. The other steps involved in IVF treatment are as follows: (4)

    Step 1: Based on the criteria of the menstrual cycle, the fertility specialists help patients to identify Day 1. The treatment begins on the first day of a woman’s periods.

    Step 2: Stimulating Medications: Medications for ovary stimulation are given to a woman for 8-14 days to increase the secretion of multiple follicles in the ovaries in order to encourage ovaries to produce more eggs. The treatment plan varies from person to person. These medications are generally inject-able and can vary from one to two injections per cycle or one to two injections per day. Most commonly, Follicle-Stimulating Hormones (FSH) and Luteinizing Hormone (LH) are given for stimulation. During this period of stimulation, a woman undergoes several blood work and ultrasounds to ascertain the size of follicles. The ideal size of follicles is considered to be 18-22 millimeters in diameter. When the follicles attain this size, this means they can be harvested. (5)

    Step 3:Egg Retrieval Process: The egg retrieval procedure is a minimally invasive surgical procedure that lasts up to a duration of 45 minutes. A woman is given human chorionic gonadotropin (HCG) shot before the planned egg retrieval. Sperm samples are collected from the male partner or donor. The HCG shot is given in order to stimulate the eggs’ release from the walls of the follicles. This is also known as trigger shots. The retrieved eggs are taken to the fertility lab for fertilization. 

    Step 4: Fertilization Process: After a successful egg retrieval process, the harvested eggs are placed into lab dishes where they are counted and tested. After six to seven hours of the egg retrieval process, in some cases, the fertilization process takes place by placing sperms onto each egg. While in other cases, the process is done with the help of another Assisted Reproductive Technique procedure, Intracytoplasmic Sperm Injection (ICSI). ICSI is a process where a selected single sperm is injected into an egg. After 15-18 hours of retrieval, fertilization of eggs is evaluated. (6)

    Step 5: Embryo Transfer: The developed or prepared embryos are then transferred into the uterus. This procedure takes place generally after three to five days of egg retrieval. The Infertility specialist determines the ideal day to perform the process of embryo transfer. The process is a simple procedure like that of Pap smear. No anesthetics are needed for this process and the process usually lasts for 5 minutes. Embryo implantation is an important factor for a successful conception therefore in some cases; certain estrogen supplements and nutritional supplements are prescribed to the patients to improve the lining of a woman’s uterus or the endometrium lining. Endometrium lining is where prepared embryo implants during the initial days of pregnancy. The fertility specialist measures the thickness of uterine lining, before performing embryo transfer. 

    Performing a Pregnancy Test

    After a successful embryo transfer procedure is performed, a blood test is performed generally after 10-14 days of the transfer to obtain accurate results. The test is carried out to evaluate the levels of HCG (human chorionic gonadotropin) hormone. Presence of HCG level, which is above 25 mIU/mL means a positive pregnancy Test. This is an IVF timeline from start to finish. The exact or accurate day for the blood work is determined by the fertility specialists based on different patients. (7)

    IVF Treatment Cost

    The average cost of IVF treatment lies between Rs. 200000- Rs300000 per conventional cycle. IVF treatment process is considered to be expensive as it involves certain medical and surgical procedures and in some cases, more than three IVF cycles are needed to perform to obtain high success rates of the procedure. (8)

    IVF Success Rates

    Over the years, the success rates of IVF procedures have increased. The success rates of pregnancy with women who have completed one IVF cycle are said to be 52 percent for women under 35. For women who are between 35-37, the success rates are 7 percent for women. The success rates of IVF procedures and pregnancy depend on various factors such as age, qualification of a doctor, location of fertility hospital and number of IVF cycles performed. (9)

    In Vitro Fertilization, (10) also known as test tube babies have helped a lot of people having their own child. Infertility is a common problem, couples facing these days due to several reasons including lifestyle. In many cases, IVF procedures are carried out along with other ART procedures such as MESA, TESA, and ICSI. To know more about IVF treatments, IVF treatment process and success stories of IVF, book a free appointment at or talk to the fertility specialists at +918268260808.



    1 Comment


    Som Prasanta

    Feb. 21, 2019, 1:05 p.m. 4.5

    Leave a Comment



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